{"id":146,"date":"2020-11-19T21:05:48","date_gmt":"2020-11-19T21:05:48","guid":{"rendered":"https:\/\/epimvp.med.umich.edu\/?post_type=creative_projects&#038;p=146"},"modified":"2021-03-03T21:30:07","modified_gmt":"2021-03-03T21:30:07","slug":"human-epilepsy-tools-core","status":"publish","type":"creative_projects","link":"https:\/\/epimvp.med.umich.edu\/research\/human-epilepsy-tools-core\/","title":{"rendered":"Human Epilepsy Tools Core"},"content":{"rendered":"\n<div class=\"max-w-xl px-4 mx-auto my-20 content text-section\">\n  <div class=\"text-lg\"><h2>Milestones<\/h2>\n<p><span style=\"font-weight: 400;\">Milestones for the core include generating stem cells with targeted gene mutations and transgenes in hPSCs using CRISPR-based methodologies, the development of iPSC lines from epilepsy patients for disease modeling and VUS studies and the differentiation of these human stem cells into neural progenitors, neurons or cortical organoids for the <em>in vitro<\/em> testing of VUS pathogenicity.<\/span><\/p>\n<\/div>\n<\/div>\n\n\n<div class=\"max-w-xl px-4 mx-auto my-20 content text-section\">\n  <div class=\"text-lg\"><p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-245 size-full\" src=\"http:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-1.jpg\" alt=\"A researcher does work in a clean lab\" width=\"583\" height=\"324\" srcset=\"https:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-1.jpg 583w, https:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-1-300x167.jpg 300w\" sizes=\"(max-width: 583px) 100vw, 583px\" \/><\/p>\n<\/div>\n<\/div>\n\n\n<div class=\"max-w-xl px-4 mx-auto my-20 content text-section\">\n  <div class=\"text-lg\"><p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-246 size-full\" src=\"http:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-3.jpg\" alt=\"A microscope image and a data visualization are stacked on top of one another.\" width=\"583\" height=\"913\" srcset=\"https:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-3.jpg 583w, https:\/\/epimvp.med.umich.edu\/wp-content\/uploads\/2020\/11\/HETC-figure-3-192x300.jpg 192w\" sizes=\"(max-width: 583px) 100vw, 583px\" \/><\/p>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>The Human Epilepsy Tools Core (HETC) functions as an independent unit housed with the Human Stem Cell and Gene Editing (HSCGE) core at the University of Michigan.  The HETC provides critical reagents and protocols for the epilepsy-focused project of the U54 including patient-derived stem cells, CRISPR-generated stem cells models and expression vectors to characterize the variants.  The HETC personnel interact closely with personnel from the GVCC and the three projects to facilitate studies of the epilepsy genes and variants.<\/p>\n","protected":false},"featured_media":241,"template":"","acf":[],"_links":{"self":[{"href":"https:\/\/epimvp.med.umich.edu\/wp-json\/wp\/v2\/creative_projects\/146"}],"collection":[{"href":"https:\/\/epimvp.med.umich.edu\/wp-json\/wp\/v2\/creative_projects"}],"about":[{"href":"https:\/\/epimvp.med.umich.edu\/wp-json\/wp\/v2\/types\/creative_projects"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/epimvp.med.umich.edu\/wp-json\/wp\/v2\/media\/241"}],"wp:attachment":[{"href":"https:\/\/epimvp.med.umich.edu\/wp-json\/wp\/v2\/media?parent=146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}